XTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL



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XTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL



TABLE OF CONTENT
CONTENT                                                                            
Title page                                                                              
Declaration                                                                                              
Certification                                                                                     
Dedication                                                                                                
Acknowledgment                                                                                    
Table of content                                                                            
Abstract                                                                                           

CHAPTER ONE
1.1    INTRODUCTION                                                                  
LITERATURE REVIEW                                                                 
1.2    TYPES OF OIL                                                                     
1.2.1 Drying oil                                                                                
1.2.2 Semi-Drying Oil                                                                     

NON- DRYING OIL                                                    

1.3    COMPOSITION OF OIL                                                      
1.4    PIGMENT                                                                          

VITAMINS                                                                                        ANTIOXIDANTS                                                                            

1.7    BOTANIC DESCRIPTION                                                   

1.8    FUNCTIONAL USES                                                           

CHAPTER TWO
MATERIALS AND METHODS                                                     
2.0    SAMPLE COLLECTION                                                      
2.1    OIL EXTRACTION                                                               
2.2    DETERMINATION OF SPECIFIC GRAVITY                    
2.3    DETERMINATION OF DENSITY                                       
2.4    DETERMINATION OF FREE FATTY ACID
CONTENT                                                                             

    1. DETERMINATION OF SAPONIFICATION VALUE          
    2. DETERMINATION OF IODINE VALUE                           
    3. DETERMINATION OF PEROXIDE VALUE                     
    4. ANTI-MICROBIAL ACTIVITY SCREENING                     
      1. REAGENTS AND MEDIA                                             
      2. PREPARATION OF FUNGAL TEST ORGANISM           
      3. PREPARATION OF THE SENSITIVE TEST AGAR                  
      4. PREPARATION OF THE NUTRIENT DEXTROSE

AGAR                                                                                    
2.8.5 THE PUNCHED AGAR DIFFUSION METHOD AS
RECOMMENDED BY BRYANT (1972)                             
2.7.6 BACTERIAL INOCULATION AND INCUBATION            
2.8.7 FUNGAL INOCULATION AND INCUBATION                            

READING INHIBITION ZONES OF THE OIL                    

CHAPTER THREE
TABLE 3.1 ORGANOLEPTIC CHARACTERISTICS OF
Crysophylum albidum SEED OIL                                                 
3.2    CHARACTERIZATION OF THE SAMPLE                        
TABLE 3.3 RESULT OF ANTI-BACTERIAL ACTIVITY OF C.
albidum OIL ON TWO GRAM POSITIVE BACTERIA                
TABLE 3.4 RESULT OF ANTI-BACTERIAL ACTIVITY OF C.
albidum OIL ON TWO GRAM NEGATIVE BACTERIA              
TABLE 3.5 RESULT OF ANTI-FUNGAL ACTIVITY OF C. albidum
OIL ON TWO TEST FUNGI                                                          
3.6    RESULT OF MINIMUM INHIBITORY CONCENTRATION
(MIC) OF C. albidum OIL ON SIX TEST ORGANISM                

CHAPTER FOUR
4.0    DISCUSSION                                                                     
4.1    CONCLUSION                                                                   

    1. RECOMMENDATION                                                           
REFERENCES                                                                               
APPENDIX I    

ABSTRACT

Crysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50and A. flavons  0.40.

The pharmacological screening confirmed the medical value of this plant oil and it established a good support for the sample in herbal medicine and as a base for the development of new drugs and phytomedicine.


CHAPTER ONE

1.1   INTRODUCTION

Oil belongs to the body of lipids which is a broad group of naturally occurring hydrophobic molecules that contain carbon. They include fats, waxes, sterols, fats-soluble vitamins, etc. This is divided into saturated and unsaturated fatty acid. Saturated fatty acids are known as fats usually obtained from animals while oil belonging to the unsaturated group is usually gotten from plants.

Crysophyllum albidum (White Star Apple) is a plant grown in the mid-Africa particularly in Nigeria, Cameroon, etc. Crysophyllum albidum (G. Don) is a tropical edible fruit tree. It belongs to the family sapotaceae which has up to 800 species and make up almost half the order Ebernales[1]. Okigbo[2] and Okafor[3] reported that a few species of Crysophyllum grow in the boarder regions between the forest and Savanna in Nigeria. The plants as reported by Bada[4], can be found in Nigeria, Cameroon, Cote d’ Ivoire, Uganda and Niger republic. The species is called different names depending on the locality. The table below shows some locality and names of the species.

 

SOME LOCALITIES AND THE SPECIE’S NAMES.

LOCALITY

 

NAME

Igbo

Yoruba

Edo

Scientific Name

 

 

 

 

Udala

Agbalumo

Otien

Chrysophyllum albidum (G.Don)

 

Flowering is usually from April to June. The flowers are sessile and occur in clusters in the leaf axils of the fruiting branch. The fruits are normally January to March but the fruit have been seen recently in November.


Fruiting branch of C. albidum


When ripe, the fruits are pale orange, edible, ovoid in shape and pointed at the apex. It is a berry with crescent shaped seeds. See the diagram below.

 

 

C. albidum Fruits with revealed seeds.


C. albidum fruit contains 8.8% protein, 17.1% oil, 21% sugar 11% starch [5]. The fruits are generally eaten by both old and young people. C. albidum fruits have been reported to be the highest source of ascorbic acid, excellent source of vitamins, irons, flavour to diets and raw material to some manufacturing industries [6]. C. albidum has been classified by Okafor [3] and Okigbo [2] as a wild uncultivated fruit tree which occurs naturally in the high forests or bushes and seldom planted as a fruit tree. Boys, girls and women generally pick C. albidum fruits from the wild forest for their consumption and sales. C. albidum fruit is a great source of economic empowerment to rural dwellers. This is because a fruit is sold at N5 (Five naira), N10 (Ten naira) depending on the size of the fruit. The anti-microbial effect of African star apple (C. albidum) belonging to the family sapotaceae is that the roots and leaves are used for medicinal purposes[7]. The seeds are used for local games[4] or discarded. The seeds of C. albidum are usually thrown away and no report has existed on the use of oils from non-utilized oil seeds for the treatment of wounds or as skin ointments.


OBJECTIVES OF THE RESEARCH

The objectives of this research work are;

  1. To extract and characterize the oil obtained from C. albidum seed.
  2. To determine the antifungal/antibacterial activity of C. albidum seed oil.

Citation - Reference

All Project Materials Inc. (2020). XTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL. Available at: https://researchcub.info/department/paper-2295.html. [Accessed: ].

XTRACTION, CHARACTERIZATION AND ANTI MICROBIAL SCREENING OF WHITE STAR APPLE (Crysophyllum Albidum) SEED OIL


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Crysophylum albidum oil was extracted from its seed. The percentage yield was 2.56%. The characterization of the oil showed showed that the refractive index is 1.487, peroxide value is 45.4mg/kg, iodine value is 50.76g, saponification value is 105.188, free fatty acids 47.46 and acid value is 94.92. The Punched Ager Diffusion Method was used to assay for the antimicrobial and anti fungal properties of the oil in the test isolate. The antimicrobial and anti fungal activity showed some inhibitory effects against test organisms; Staphylococcus aureus, E. coli, B. subfilis, C. albican and A. flavons, but non for S. pyogens. The minimum inhibitory concentration of these test organisms are as follows; Staphylococcus aureus0.16, E. coli 0.06, B. subfilis 0.14, C. albican 2.50and A. flavons 0.40... Click here for more

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