GENETIC DIVERSITY OF FIVE POPULATIONS OF THE NIGERIAN LOCAL BREEDS OF GOAT USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS


GENETIC DIVERSITY OF FIVE POPULATIONS OF THE NIGERIAN LOCAL BREEDS OF GOAT USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

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GENETIC DIVERSITY OF FIVE POPULATIONS OF THE NIGERIAN LOCAL BREEDS OF GOAT USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

ABSTRACTS

Genetic diversity and genetic distance of five populations of the Nigerian breeds of goat were

investigated using random amplified polymorphic DNA markers. Five breeds of goat were

used for the study and each breed constituted a population, hence, the five populations

considered in the study. The populations were: Sokoto Red (SR), Sahel (SH), Kano Brown

(KB), Bornu White (BW) and West African Dwarf (WAD) goats. The experiment was

conducted within four geographical zones of Nigeria: South east, North west, North east and

North central. One hundred and twenty (120) blood samples were randomly collected from

various locations across the four geographical zones in Nigeria. The samples were collected

from the stocks at the central markets, which served as collection point from all the localities

and from household goat keepers.

 

Varying numbers of sample sizes (Sokoto Red = 23, Kano

Brown = 21, Bornu White = 23, WAD = 26 and Sahel = 27) were collected from each breed.

The blood samples were collected from the jugular vein of the animals through a process

known as venipuncture. Approximately, 5 ml of blood was collected aseptically from each

animal into an EDTA container, using 23 gauge sterile needle and syringe, and was stored at

-20oC using (ethylene-di-amine-tetra-acetic acid, EDTA) as anticoagulant containers. The

DNA samples were isolated and purified from the 120 blood samples following protocol as

recommended by (ZYMO RESEARCH CORPORATION, e-mail: The RAPD-PCR reaction followed the procedure described by El Hentati et al., (2012). Seven random primers were used for this study but only three (3) primers produced clearly polymorphic and reproducible bands whereas four (4) failed to

produce any band. Allele frequency per population per loci under consideration exceeded the

minimum allele frequency (MAF) limit 10%. Level of polymorphism per primer varied from

43 – 80%. Total of 20 scoreable bands were obtained and thirteen (13) out of which were

polymorphic to arrive at a total of 65% polymorphism. Genetic diversity of the studied

populations was measured with three indices: (Nei’s genetic diversity, Shannon’s

information index, Observed and Effective number of alleles). Observed number of allele

(Na) was 2.0000 across the populations while Effective number of alleles (Ne) varied from

1.9003 in WAD to 1.9900 in Sahel population. However, SR, KB and BW had Ne values of

1.9897, 1.9287 and 1.9836 respectively. Nei’s heterozygosity varied across the populations

with highest values 0.4975 and 0.4974 obtained in Sahel and Kano Brown respectively

whereas lowest value 0.4736 was obtained in WAD. Shannon’s Information index followed

similar trend across with average of 0.6822.

 

The mean of coefficient of gene differentiation

Gst was (0.0139) and mean of gene flow Nm across populations was (35.3710). The highest

genetic similarity (0.9995) and lowest genetic distance (0.0005) was recorded between

Sokoto Red and Sahel, while the lowest similarity (0.9505) and highest genetic distance

(0.0507) was recorded between Bornu White and WAD. Populations with higher similarity

indicated that they are of closer descent and closer geographical locations. The unweighted

pair group method of arithmetic means (UPGMA) dendrogram based on Nei’s genetic

distance clearly separated the five populations into two clusters. The closest relationship was

observed between Sokoto Red, Sahel and Bornu White goat populations/breeds and the

farthest relationship was observed between WAD and Sokoto Red populations. It was

concluded that the high similarity obtained in this study was as a result of loss of

heterozygosis in the populatons of Nigerian breeds of goat which may have originated

among mates in each population/breed. However, the results of this experiment can offer

some crucial scientific data useful for breeding programme of Nigerian breeds of goats

GENETIC DIVERSITY OF FIVE POPULATIONS OF THE NIGERIAN LOCAL BREEDS OF GOAT USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

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A Research proposal for genetic diversity of five populations of the nigerian local breeds of goat using random amplified polymorphic dna (rapd) markers:
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Genetic diversity and genetic distance of five populations of the Nigerian breeds of goat were investigated using random amplified polymorphic DNA markers. Five breeds of goat were used for the study and each breed constituted a population, hence, the five populations considered in the study. The populations were: Sokoto Red (SR), Sahel (SH), Kano Brown (KB), Bornu White (BW) and West African Dwarf (WAD) goats. The experiment was conducted within four geographical zones of Nigeria: South east, North west, North east and North central. One hundred and twenty (120) blood samples were randomly collected from various locations across the four geographical zones in Nigeria. The samples were collected from the stocks at the central markets, which served as collection point from all the localities and from household goat keepers. Varying numbers of sample sizes (Sokoto Red = 23, Kano Brown = 21, Bornu White = 23, WAD = 26 and Sahel = 27) were collected from each breed. The blood samples were collected from the jugular vein of the animals through a process known as venipuncture. Approximately, 5 ml of blood was collected aseptically from each animal into an EDTA container, using 23 gauge sterile needle and syringe, and was stored at -20oC using (ethylene-di-amine-tetra-acetic acid, EDTA) as anticoagulant containers. The DNA samples were isolated and purified from the 120 blood samples following protocol as recommended by (ZYMO RESEARCH CORPORATION, e-mail: The RAP.. agricultural extension project topics

GENETIC DIVERSITY OF FIVE POPULATIONS OF THE NIGERIAN LOCAL BREEDS OF GOAT USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

Project Information

  • CATEGORY : AGRICULTURAL EXTENSION
  • TYPE : PROJECT MATERIAL
  • FORMAT : MICROSOFT WORD
  • ATTRIBUTE : Documentation Only
  • PAGES : 53 Pages
  • CHAPTERS : 1 - 5
  • PRICE : ₦ 3,000.00

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