ABSTRACT
Anemia is a serious health problem especially in developing
countries. In the tropics, rural dwellers had resort to herbal treatment
in some cases of anemia. The main aim of this work was to ascertain the
role of n-butanol leaf fraction of Telfairia Occidentalis on some
haematological parameters in phenyl hydrazine induced anemia in wistar
rats. Thirty (30) adult Wistar rats were used for this study. All
animals were assayed for haematological parameters 1day before the onset
of the experiment. The animals were grouped into five groups of five
rats each (n =5). Anemia was induced in the Wistar rats by
intraperitoneal injection of Phenyl hydrazine Hydrochloride 50mg/kg w/w
in DSMO once daily for 3 days. Group i. serves as the negative control
group, received 50mg/kg of Phenylhydrazine Hydrochloride and 1ml/kg
normal saline (untreated) group ii. serve as Positive control group
received Vitamin B12 0.4ml/kg (standard haematinic)
intramuscularly,while group iii, iv. and v. received 100mg/kg, 200mg/kg
and 300mg/kg body weight of n-butanol leaf fraction of Telfairia occidentalis for 2
weeks respectively. Asessment of RBC and WBC was carried out using the
newly improved Neubauer counting chamber, PCV using the microhaematocrit
reader, Hb using the AO-Hb meter and Bilirubin concentration using
colorimetric estimation for the serum bilirubin. There was a significant
(P<0 .05="" after="" and="" as="" bilirubin="" compared=""
concentration="" control="" fraction="" groups="" hb="" in=""
increase="" level="" of="" pcv="" rbc="" respectively.="" span="" the=""
to="" treatment="" with="" x10="">6/ʯL as compared to
4.78±0.17x106/ʯL), PCV (50.60±0.51% as compared to 37.08±0.37%), HB
(16.84±0.18 g/dl as compared to 12.56±0.13 g/dl), WBC (6.10±0.15 x109/L
as compared to 6.17±0.88x109/L) and Bilirubin (18.16±0.08a ʯmol/L as
compared to 15.54±0.21 ʯmol/L). However, the significant increase
obtained from the results of RBC ,PCV and Bilurubin was not dose
dependent while that of HB and WBC was dose dependent. In conclusion,
Intraperitoneal administration of 50mg/kg Phenylhydrazine hydrochloride
for 3days decreases the blood parameters below the pre-anemic level
(1day before induction) due to the production of reactive oxygen
species. Phytochemical screening of this leaf fraction indicates the
presence of flavonoids, saponin secoiridoid glycosides and alkaloids,
these natural antioxidants could be responsible for reversing the
damaging effect of PHZ and thus playing a modulatory role and also
maintaining the integrity of the RBC.
CHAPTER ONE 1.0 INTRODUCTION
Anemia is a global public health problem affecting both developing
and developed countries with major consequences for human health as well
as social and economic development (WHO, 2005).
Anemia is a reduction from the normal quantity of circulating
hemoglobin in the blood less than 13 g/dl for male and less than 12 g/dl
for female adults (Okochi et al., 2003). It occurs at all stages of the life cycle, but is more prevalent in pregnant women and young children (Adam et al., 2005).
Hemolytic anemia is a form of
anemia due to
hemolysis, the abnormal breakdown of
Red Blood Cells (RBCs), either in the
blood vessels (intravascular
hemolysis) or elsewhere in the human body (extravascular). It has
numerous possible causes, ranging from relatively harmless to
life-threatening (Telford
et al., 2003). The general classification of hemolytic anemia is either
inherited or acquired. Treatment depends on the cause and nature of the breakdown (Yamoto
et al., 1998).
Anemia is the result of a wide variety of causes that can be
isolated, but more often coexist. Globally, the most significant
contributor to the onset of anemia is iron deficiency so that IDA and
anemia are often used synonymously, and the prevalence of anemia has
often been used as a proxy for IDA (De Maeyer, 1989). It is generally
assumed that 50% of the cases of anemia are due to iron deficiency, but
the proportion may vary among population groups and in different areas
according to the local conditions (De Maeyer, 1989).