THE EFFECT OF CRUDE OIL CONTAMINATED SOIL ON THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF BEANS (PHASEOLUS VULGARIS)


THE EFFECT OF CRUDE OIL CONTAMINATED SOIL ON THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF BEANS (PHASEOLUS VULGARIS)

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THE EFFECT OF CRUDE OIL CONTAMINATED SOIL ON THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF BEANS (PHASEOLUS VULGARIS)

LIST OF FIGURES

Figure 2.1: Chemical structure of some polyphenolic compounds present in common beans      23

Figure 2.2: Initiation step of lipid peroxidation                                                                              38

Figure 2.3: Initial phase of propagation step of lipid peroxidation process indicating the             39

Oxygen uptake

Figure 4.1: Effect of crude oil-impacted soil on height of beans over a period of nine                 63

(9) weeks

Figure 4.2: Effect of crude oil-impacted soil on stem girth of beans over a period of nine           64

(9) weeks.

Figure 4.3: Effect of crude oil-impacted soil on the relative water content (RWC) of planted     65

Beans over a period of nine (9) weeks

 

 

 

 

 

 

LIST OF TABLE

Table 2.1: Characteristic of plant ascorbate peroxidase                                                                 42

Table 4.1: Effect of crude oil impacted soil on activities of superoxide dismutase (SOD),         66

Catalase (CAT), peroxidase (POD), ascorbate perioxidase (APX) and glutathione reductase

(GR) activities of beans leaves.                                              

Table 4.2: Effect of crude oil impacted soil on activities of superoxide dismutase (SOD),          67

Catalase (CAT), peroxidase (POD), ascorbate perioxidase (APX) and glutathione reductase

(GR) activities of beans stems.

Table 4.3: Effect of crude oil impacted soil on soluble protein content (SPC)                              68 and malondialdehyde (MDA) of beans leaves.

Table 4.4: Effect of crude oil impacted soil on soluble protein content (SPC)                              69   and malondialdehyde (MDA) of beans stems.

 

 

 

 

 

 

ABSTRACT

The effect of crude oil contaminated soil on physical and biochemical properties
of beans (
phaseolus vulgaris) was investigated. Fifteen polythene pots with drainage holes at the bottom, each containing 10 kg of surface soil, were randomly placed on a table in the screenhouse in a factorial combination of five treatment levels (0.4%, 0.3%, 0.2%, 0.1% and 0% w/w) of crude oil and were designated P4, P3, P2, P1 and P0
respectively. Three seeds of beans per pot were planted. Growth parameters (plant height, stem girth, relative water content (RWC), and soluble protein content (SPC)) and antioxidant indices were determined in the beans over a period of nine weeks after planting (WAP). Results showed that growth of beans planted in contaminated soil was significantly lower (p<0.05) than that of control. Beans planted in the contaminated soil also showed a significant increase (p<0.05) in superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR) activities in leaves when compared with control plants. The results suggest that crude oil contaminated soil hindered availability of water, air and
nutrients to beans roots, creating a drought condition which could induce oxidative stress in the plant and consequently retarding growth and yield of beans plant.

 

 

 

 

 

 

 

 

CHAPTER ONE

1.0    INTRODUCTION

Crude oil is a complex mixture of thousands of hydrocarbons and non-hydrocarbon compounds, including heavy metals. Nigeria is an established crude oil exporting nation producing medium and light crude oil, such as bonny light (Amund et al., 1993). Crude oil exportation is the main stay of Nigeria’s economy. Crude oil is a colloidal mixture of different hydrocarbons (90%) and non-hydrocarbon (10%) components (Cadwellaer et al., 1993). Various activities in crude oil exploration, exploitation, storage and transportation lead to spillage of oil to the environment (Agbogidi et al., 2007). Crude oil causes harmful effects on the environment, where it poses a serious threat to organisms and farmland that are linked in a complex food chain that includes humans (Lundstedt et al., 2003). The effects of crude oil on the growth and performance of plants have been reported in many studies (Njoku et al., 2008). Crude oil in soil makes the soil condition unsatisfactory for plant growth. It can reduce the level of available plant nutrient in contaminated soils (Jong et al., 1980) and can also raise the levels of certain elements such as iron and zinc to toxic amounts (Udo et al., 1975). Water and oil are usually considered to be immiscible. However, crude oil contains a very small soluble portion referred to as the water soluble fraction (WSF). The soluble constituents are dispersed particulate oil, dissolved hydrocarbons and soluble contaminants such as metallic ions. The components of crude oil that go into solution make up the WSF. The lower the molecular weight of the constituent hydrocarbon of crude oil, the higher is its concentration in the water-soluble fraction (Edema et al., 2012).

Exposure of plants to crude oil and heavy metal poisoning has been reported to produce reactive oxygen species (ROS) and other free radicals which induce oxidative stress and cause lipid peroxidation (Blokhina et al., 1999). Even at an early stage, it can cause a reduction in cell proliferation and growth. Various researchers have reported activation of lipid peroxidation in plants exposed to different pollutants (Chirkova et al., 1998). Increase in superoxide dismutase (SOD) activity has also been reported in oat, wheat and Arabidopsis thaliana in soils contaminated with various pollutants (Alscher et al., 2002). Several plant species have also been shown to have elevated peroxidase activities in response to increased pollutant concentrations (Jouili et al., 2003). Reactive oxygen species is thought to increase cellular damage through the oxidation of several macromolecules such as lipids and proteins (Ortega et al., 2005).

The common dry beans or Phaseolus vulgaris L., is the most important food legume for direct consumption in the world. Among major food crop, it has one of the highest levels of variation in growth habit, seed characteristics (size, shape, colour), maturity, and adaptation. It also has a tremendous variability (> 40,000 varieties). In Africa, beans are grown mainly for subsistence, where the Great Lakes region has the highest per capita consumption in the world. Beans are a nearly “perfect” food. Nutritionally rich, they are also a good source of protein, folic acid, dietary fibre and complex carbohydrates. Beans are also one of the best non-meat sources of iron, providing 23-30% of daily recommended levels (Pachico et al., 1993) from a single serving. Consumption of beans is high mostly because they are a relatively inexpensive food. For the poor of the world, they are a means of keeping malnutrition at bay (WHO. 1992).

This research work is geared towards outlining the effect of soil contaminated with crude oil (at various levels) on the biochemical properties and growth rate of beans (phaseolus vulgaris).

THE EFFECT OF CRUDE OIL CONTAMINATED SOIL ON THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF BEANS (PHASEOLUS VULGARIS)

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A Research proposal for the effect of crude oil contaminated soil on the physical and biochemical properties of beans (phaseolus vulgaris):
A Review on the effect of crude oil contaminated soil on the physical and biochemical properties of beans (phaseolus vulgaris), effect, crude, contaminated project topics, researchcub.info, project topic, list of project topics, research project topics, journals, books, Academic writer.
The effect of crude oil contaminated soil on physical and biochemical properties of beans (phaseolus vulgaris) was investigated. Fifteen polythene pots with drainage holes at the bottom, each containing 10 kg of surface soil, were randomly placed on a table in the screenhouse in a factorial combination of five treatment levels (0.4%, 0.3%, 0.2%, 0.1% and 0% w/w) of crude oil and were designated P4, P3, P2, P1 and P0 respectively. Three seeds of beans per pot were planted. Growth parameters (plant height, stem girth, relative water content (RWC), and soluble protein content (SPC)) and antioxidant indices were determined in the beans over a period of nine weeks after planting (WAP). Results showed that growth of beans planted in contaminated soil was significantly lower (p<0.05) than that of control... soil science project topics

THE EFFECT OF CRUDE OIL CONTAMINATED SOIL ON THE PHYSICAL AND BIOCHEMICAL PROPERTIES OF BEANS (PHASEOLUS VULGARIS)

Project Information

  • CATEGORY : SOIL SCIENCE
  • TYPE : PROJECT MATERIAL
  • FORMAT : MICROSOFT WORD
  • ATTRIBUTE : Documentation Only
  • PAGES : 130 Pages
  • CHAPTERS : 1 - 5
  • PRICE : ₦ 3,000.00

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