ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCocos nucifera(COCONUT) WATER AND METHALONIC EXTRACT OF THE YOUNGCocos nucifera(COCONUT) HUSK

ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCocos nucifera(COCONUT) WATER AND METHALONIC EXTRACT OF THE YOUNGCocos nucifera(COCONUT) HUSK


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ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCocos nucifera(COCONUT) WATER AND METHALONIC EXTRACT OF THE YOUNGCocos nucifera(COCONUT) HUSK

PROJECT TOPICS AND MATERIALS ON ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCocos nucifera(COCONUT) WATER AND METHALONIC EXTRACT OF THE YOUNGCocos nucifera(COCONUT) HUSK


CHAPTER ONE

1.0
INTRODUCTION AND LITERATURE REVIEW

1.1 INTRODUCTION 

Medicinal  plants  represent  a  rich  source  of  antimicrobials  and  many  other  drugs.  The  potentialsof

higher  plants  as  source  for  new  drugs  is  still  largely  unexplored.  Antibiotic  resistance  has  become  a

global  concern  (Westh
et  al., 2004).  The  clinical  efficacy  of  many  existing  antibiotics  is  being

threatened  by  the  emergence  of  multidrug-resistant pathogens  (Bandow,  2003).  Many

infectiousdiseases  have  been  known  to  be  treated  with  herbal  remedies  throughout  the  history  of

mankind.  Natural  products,  either  as  pure  compounds  or  as  standardized  plant  extracts,  provide

unlimited opportunities for new drug leads because of the unmatched availability ofchemical diversity.

There is a continuous and urgent need to discover new antimicrobial compounds with diversechemical

structures  and  novel  mechanisms  of  action  for  new  and  re-emerging  infectious  diseases  (Rojas  et  al.,

1992).  Therefore,  researchers  are  increasingly  turning  their  attention  tolocal  herbs,  looking  for  new

leads todevelop better drugs against microbial infections (Benkeblia, 2004). 

The increasingfailure of chemotherapeutics and antibiotic resistanceexhibited by pathogenic microbial

infectious  agents  has  led  to  the screening  of  several  medicinal  plants  for their  potential  antimicrobial

activity  (Kapila, 2005 ;Runyoro  et  al.,2006). The rising prevalence of  antibiotics  resistant pathogenic

microorganisms raises the demand for finding new alternative antimicrobial agents. The drugs already

in use to treat infectious diseaseare of concern because drug safety remains an enormous global issue.

Most of the synthetic drugscause side effects and also most of the microbesdeveloped resistant against

the synthetic drugs (Chanda and Rakholiya 2011). To alleviate this problem, antimicrobial compounds

frompotential plants should be explored. These drugs fromplants are less toxic; side effects are scanty

and  alsocost  effective.  They  are  effective  in  the  treatment  ofinfectious  diseases  while




                                          1
 
simultaneouslymitigatingmany of the side effects that are often associated withsynthetic antimicrobials

(Harishchandra
et al., 2012).

 Published studies in medical journals show that  coconut in one form or  another  may provide a  wide

range  of  health  benefits.  The  coconut  plant
Cocos  nucifera (family  Arecaceae)  is  considered  as  an

important  fruit  crop  in  tropical  countries.  It  is  commonly  available  plant  with  wide  variety  of

applications in food, drinks,  fibers, building  materials and various  chemicals finding  their way into a

huge  range  of  modern  day  products.  Being  highly  nutritious  coconuts  have  also  been  studied  for

medicinal qualities. 

Modern medical science is now confirming the  medicinal qualities of
Cocos nucifera  which are  used

for the treatment wide range of infections. Based on the knowledge of the traditional herbs used for the

treatment  for  local  application,  coconut  husk  can  be  use  as  a  topical  antimicrobial.  As  preliminary

investigation of the use of coconut husk, the antimicrobial activity can be evaluated. 

1.2 AIM AND OBJECTIVES

The specific objectives are to:


(a)  Evaluate  the  phytochemical  and  antimicrobial  activities  of  Methalonic  extract  of  young
  Cocos

nucifera
husk on selected pathogenic microorganisms. 


(b)  Evaluate  the  antimicrobial  activities  and
young  Cocos  nucifera water  on  selected  pathogenic

microorganisms


1.3 JUSTIFICATION OF THE RESEARCH


Cocos nucifera husk and Cocos nucifera water are traditionally used in the treatment of wide variety of

diseases,  it  has  been  used  from  time  immemorial  for  the  treatment  of  carcinogenic  infections.  This

study scientifically justifies the use of young Coconut husk and young Coconut water in traditional folk

medicine and to compare their antimicrobial potency with the commercial antibiotics.


                                          2
 
1.4LITERATURE REVIEW

1.4.1 MEDICINAL PLANTS AS ANTIMICROBIAL AGENT


Medicinal  plants  have  always  been  considered  as  a  source  for  healthy  life  for  people.  Therapeutical

properties  of  medical  plants  are  very  useful  in  healing  various  diseases  and  the  advantage  of  these

medicinal plants are natural (Kalemba and Kunicka
, 2003). In many parts of the world, medicinal plants

have been used for its antibacterial, antifungal and antiviral activities for hundreds of years (Ali
et al.,

1998; Barbour et al., 2004; Yasunakaet al., 2005).  Researchers are increasingly turning their attention

to natural products and looking for new leads to develop better drugs against cancer, as well as viral and

microbial  infections  (Ibrahim,  1997;  Towers
et  al.,  2001;  Koshy et  al.,  2009).  Several  synthetic

antibiotics  are  employed  in  the  treatment  of  infections  and  communicable  diseases.  The  harmful

microorganisms  can  be  controlled  with  drugs  and  this  has  resulted  in  the  emergence  of  multiple  drug

resistant bacteria and it has created alarming clinical situations in the treatment of infections. 

In general, bacteria have the genetic ability to transmit and acquire resistance to synthetic drugs which

are  utilized  as  therapeutic  agents  (Murray,  1992;  Madunagu
et  al.,  2001;  Koshy et  al.,  2009;

Senthilkumar  and  Reetha
, 2009)  Therefore,  actions  must  be  taken  to  reduce  this  problem,  such  as  to

minimize the use of antibiotics, develop research of resistance among microorganism and to  continue

studies to develop new antibiotic and immune modulating compounds with diverse chemical structures

and  novel  mechanisms  of  action,  either  synthetic  or  natural  to  control  pathogenic  microorganisms

because  there  has  also  been  an  alarming  increase  in  the  incidence  of  new  and  re-emerging  infectious

diseases (Ikenebomeh and Metitiri, 1988; Rojas
et al., 2003)

Antimicrobial studies have shown that Gram-negative bacteria show a higher resistance to plant extracts

than  Gram-positive  bacteria.  This  may  be  due  to  the  variation  in  the  cell  wall  structures  of  Gram-

positive and Gram-negative bacteria. More specifically, Gram-negative bacteria has an outer membrane

that  is  composed  of  high  density  lipopolysaccharides  that  serves  as  a  barrier  to  many  environmental



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Medicinal plants represent a rich source of antimicrobials and many other drugs. The potentialsof higher plants as source for new drugs is still largely unexplored. Antibiotic resistance has become a global concern (Westhet al., 2004)... biology project topics

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